Figure 3.

Pac knockdown attenuates acid-induced cell death of primary rat cortical neurons. (a), Representative images (left) and quantification (right; mean ± SEM, n = 5 coverslips for each group, two-tailed Student’s t test) of acid-induced cell death in control and shPAC-2-treated primary neurons (1-hour pH 7.3 or 5.6 treatment and 24-hour recovery in culture medium), stained with Hoechst 33342 (blue) for total nuclei and Propidium iodide (red) for dead neurons at 20x magnification. (b), Quantification (mean ± SEM, n = 3 wells for pH 7.3 and n = 6 wells for pH 5.6, two-tailed Student’s t test) of acid-induced cell death by the percentage release of LDH from rat cortical neurons (1-hour pH 7.3 or 5.6 treatment and 24-hour recovery in culture medium), normalized to total LDH.