Figure 2.

Alveolar macrophages can uptake osteosarcoma cell exosomes. Osteosarcoma cell exosomes were extracted from non-metastatic K7 and Dunn cells and from metastatic K7M3 and DLM8 cells via ultracentrifugation and then labeled with Cell Tracker CM-DiI red fluorescent dye. Alveolar macrophages were cultured overnight and then treated with labeled osteosarcoma cell exosomes for 24 h. Both fluorescent and bright field images were collected using the (a) IncuCyte Live-Cell System at 20× and the (b) Nikon Eclipse Ti de-convolution inverted bright field and fluorescent microscope at 60× under oil immersion. Osteosarcoma cell exosomes are shown in red and DAPI in blue. Bright field images are also included, as well as the merged images from all three channels.