Skip to main content
. 2020 Apr 12;9(1):1747677. doi: 10.1080/2162402X.2020.1747677

Figure 6.

Figure 6.

Induction of TGFB2 by exosomes from K7M3 and DLM8 metastatic cells plays a role in inhibiting macrophage function. Alveolar macrophages (MHS cells) were cultured overnight and then incubated with exosomes from osteosarcoma cells (non-metastatic K7 and Dunn; metastatic K7M3 and DLM8) for 48 h. IL10 (a) and TGFB2 (b) secretion in culture medium was measured by ELISA. *** indicates a p vale < 0.0005 (c,d) Alveolar macrophages (MHS cells) were cultured overnight and then incubated with exosomes from metastatic K7M3 (c) cells and DLM8 (d) cells with or without TGFB2 antibody (0.1ug/mL) for 24 h. Osteosarcoma cells, labeled with IncuCyte pHrodo labeling reagent, were then added to the MHS cell culture. Phagocytic activity was determined using the IncuCyte S3 Live-Cell Analysis system and the data analyzed by IncuCyte software. Significant inhibition (p < .05) in phagocytic activity was seen at twelve hours for the MHS cells incubated with both K7M3 and DLM8 exosomes as compared to control. There was no significant differences observed at twelve hours between control and K7M3 or DLM8 exosomes plus anti-TGFB2.