Table 1.
Deamidation in Combo, mAb-A and mAb-B fractions by HIC, and mAb-A and mAb-B.
| Deamidation (%) |
||||
|---|---|---|---|---|
| Condition | Sample Description | HC Asn-388, Asn-393 | HC Asn-329 | mAb-B LC Asn-30 |
| 25°C | Combo | 4.3 | 5.3 | 18.6 |
| mAb-A | 3.5 | 5.6 | NA | |
| mAb-A fraction by HIC | 3.8 | 6.6 | NA | |
| mAb-B | 4.2 | ND | 14.3 | |
| mAb-B fraction by HIC | 3.9 | ND | 13.4 | |
| 40°C | Combo | 8.8 | 47.4 | 60.0 |
| mAb-A | 8.7 | 48.3 | NA | |
| mAb-A fraction by HIC | 8.6 | 48.3 | NA | |
| mAb-B | 6.9 | 8.8 | 33.6 | |
| mAb-B fraction by HIC | 9.2 | 10.8 | 45.9 | |
HC = heavy chain; LC = light chain; NA = not applicable; ND = not detected.