Table 2.
Observed post-translational modifications of Combo, mAb-A and mAb-B under stress conditions.
| Sample | (a) Deamidation sites under pH stress at 6.5 |
(b) Methionine oxidation sites under chemical oxidation conditions |
(c) Tryptophan oxidation sites under photostress conditions |
|||||
|---|---|---|---|---|---|---|---|---|
| HC Asn-388, Asn-393 | mAb-B LC Asn-30 | HC Met-256 | HC Met-432 | HC Met-362 | mAb-B HC Met-401 | mAb-B HC Trp-52 | mAb-A HC Trp-102 | |
| Combo | 8.8 | 31.0 | 80.0 | 38.7 | 42.2 | 55.2 | 12.2 | 61.2 |
| mAb-A | 8.2 | NA | 79.9 | 39.7 | 45.4 | NA | NA | 63.6 |
| mAb-B | 8.8 | 30.6 | 60.9 | 41.3 | 22.0 | 56.9 | 10.9 | NA |
HC: heavy chain; LC: light chain; NA = not applicable.