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. 2020 Mar 26;12(1):1742457. doi: 10.1080/19420862.2020.1742457

Figure 2.

Figure 2.

Isolation of GP-specific monoclonal antibodies. (a) Binding capacity of the serum of vaccine-immunized subjects # 024, 057, and 088 to EBOV GP, BDBV GP, and SUDV GP. Values represent the difference in optical density (OD) between sera (1:10,000) on day 28 post-boost immunization and day 0 from the same donor. See also Figure S1a. (b) Neutralizing capacity of the serum of vaccine-immunized subjects # 024, 057, and 088 against pseudotyped HIV-EBOV GP-Luc. Data on the curve represent the difference in neutralization ability between sera on day 28 post-boost immunization and day 0 from the same donor. (c) Sorting of CD3−/CD38−/IgG+/CD19+/CD27+/GP∆Muc+ single memory B cells obtained from PBMCs one month post-boost immunization to identify GP-specific mAbs. (d) Number of specific or cross-reactive antibodies identified using the supernatants of Ig genes linear expression cassettes. See also Figure S1b. (e) Correlation between GP sequence similarity to EBOV GP and number of binding antibodies. (f) Number of antibodies binding to different truncated EBOV GPs determined by ELISA using 293 T supernatants. See also Figure S1b.