Figure 6.

Influence of GP form and thermolysin cleavage on mAbs binding. (a) Binding of mAbs to EBOV GP displayed on the surface of BHK-T7 cells. Images were acquired under a 4× objective on a Cytation imaging reader. (b) and (c) Binding of mAbs to uncleaved or cleaved EBOV GP/GP∆Muc displayed on the surface of 293 T cells and analyzed using flow cytometry. Fold change in mAb binding after thermolysin cleavage was calculated by comparing the percentage of PE-positive cells with or without thermolysin (THL) digestion. See also Figure S6 and Figure S7. (d) NPC1-C binding inhibition. See also Figure S4b.