Figure 3.
(a) Mass spectrum of CD16a (L) in complex with mAb (RL) measured from 150 mM ammonium acetate solution, pH 7.4 in ESI positive mode. The red dotted line approximated by linear regression indicates the boundary of integrated area between mAb and its complex. Each measurement was repeated three times and the data obtained from titration experiments were fitted with a model equation. Saturation binding curve as a function of free ligand concentration for WT (b), G0 F (c), G2 F (d), ST3 (e) and ST6 (f) glycovariants with estimated Kd values are denoted by the red square line on an x-axis at half of the total mAb associated with CD16a.