Table 2.
The Kd determination with MST using trace analysis and initial fluorescence change. The CD16a was covalently labeled with the 2nd generation RED-NHS dye and titrated with serial dilutions of WT and glycovariants.
| MST-Trace Analysis |
Initial Fluorescence Change Analysis |
|||
|---|---|---|---|---|
| mAb | Kd [µM] | S/N | Kd [µM] | S/N |
| WT | 2.4 ± 0.6 | 7.7 ± 1.0 | 1.6 ± 0.4 | 10.9 ± 0.6 |
| G0 F | 2.2 ± 0.2 | 8.3 ± 1.2 | 0.6 ± 0.1 | 11.9 ± 1.4 |
| G2 F | 1.5 ± 0.1 | 10.2 ± 0.2 | 0.6 ± 0.1 | 19.6 ± 1.0 |
| ST3 | 2.6 ± 0.4 | 10.9 ± 1.6 | 1.1 ± 0.1 | 13.7 ± 4.1 |
| ST6 | 2.6 ± 0.1 | 8.6 ± 1.2 | 1.2 ± 0.2 | 11.6 ± 2.9 |