Figure 5.

Engaging a membrane-proximal region within MSLN increases FcγR-IIIA signaling. NCI-N87 target cells were incubated with a dose range of either CD47xMSLN bsAb#1 or bsAb#2 (a–c), then mixed with engineered FcγR-I (a), FcγR-IIA (H131 polymorphism) (b) or FcγR-IIIA (V158 polymorphism) (c) reporter Jurkat cell lines. After 6 h of co-culture at 37°C, the luciferase activity was measured. Data are then presented as fold change induction. Graphs depict a representative dose–response curve obtained for a minimum of two independent experiments tested in duplicate.