Figure 2.

Schematic illustration of protein sequences for heavy and light chains fused to split intein partners Int^C and Int^N. Protein sequences for heavy and light chains of huFc IgG, Fab and oaSEED fragments with their corresponding molecular weights are exemplarily shown used for antibody reconstitution via split inteins. The partial hinge region is depicted as amino acid sequence fused to Extein^C or Extein^N (underlined) and the corresponding split inteins Int^C or Int^N. Heavy chains fused to split intein partners were further fused to a hexahistidine tag. Heavy chains fused to Int^C were attached to a glycine-serine linker. 1: HC for a huFc Int^C fragment. 2 + 3: hu225H Fab Int^N HC and corresponding LC. 4 + 5: Reconstituted IgG with modified hinge region aligned to reference IgG. 6–9: B10v5 oaSEED Int^C with corresponding HC and LC. Detailed sequence information can be found in the supplements.