Figure 4.

CD8 TIL landscape modulation upon anti-PD-1 therapy.

(a) Gene signatures of EM-like, memory-like and exhausted derived from our scRNA-seq analysis. For visualization, 100 random cells were sampled from each TIL state, and top differentially expressed genes are displayed. Horizontal lines separate groups of genes with similar expression patterns. (b) TIL state gene-signature enrichment analysis (GSEA) for the transcriptomic response of bulk endogenous CD8 TILs to PD-1 blockade (raw from Gubin et al.²⁷). NES = Normalized Enrichment Score. (c) TILPRED classification of total (left) and PD-1^low (right) CD8 TIL from MC38 colon adenocarcinoma (datasets from Xiong et al.³⁷ and Kurtulus et al.³⁸ expression of markers in Supplemental Figure 8). (d) States composition (TILPRED classification) of CD8 TILs upon ICB in MC38 (anti-PD-1 +anti-TIM3 vs isotype control) and sarcoma (anti-PD-1 vs isotype control)