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. 2020 Apr 7;9(1):1747732. doi: 10.1080/2162402X.2020.1747732

Figure 1.

Figure 1.

CD44 and CD44v3 expression on cell lines and on exosomes produced by these cell lines. In a: Percentages of CD44+ and CD44v3+ cells in cultures of various cell lines as determined by flow cytometry. The data are means ± SD of four experiments performed with each cell line. In b: Representative flow cytometry results for CD44+ and CD44v3+ exosomes stained with labeled anti-CD44 or anti-CD44v3 Abs, respectively, and examined by on-bead flow cytometry. Note higher RFI values for CD44v3 exosomes, except in case of SSC47 exosomes. *p < .01. In c: Comparisons of RFI values for CD44+ and CD44v3+ exosomes produced by the cell lines shown in a. Note that exosomes produced by HNSCC cell lines are uniformly positive for CD44v3. The data are means ± SD of three flow cytometry assays for each exosome preparation.