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. 2020 Apr 7;9(1):1747732. doi: 10.1080/2162402X.2020.1747732

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© 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Detection of antigens carried by immunocaptured CD44v3 exosomes from plasma of HNSCC patients (n = 12). In parallel, exosomes were also captured with anti-CD45 mAb as a control. Flow cytometry-based analysis showed the highest levels of EPCAM on CD44v3(+) exosomes. Expression levels of CD44v3 and EPCAM were also high in the CD45(-) fraction enriched in TEX; they were low in CD45(+) and CD44v3(-) exosomes. CD44 expression levels were comparable in all exosome fractions (*p < .05, **p < .005). Gray bars indicate CD44v3+ or CD45+ exosomes, while white bars indicate CD44V3(-) and CD45(-) exosomes.