Table 3.
Spearman correlations (ρ > 0.5) for selected analytical characterization read-outs with p-values <0.0001 separated by isotype (IgG1 and IgG4). Pearson coefficients and associated p-values are also shown. P-values test null hypothesis that the correlation coefficient = 0.
| Variable | by Variable | Spearman ρ | P-Value | Pearson r | P-Value |
|---|---|---|---|---|---|
| IgG1 | |||||
| UP-SEC Low pH Retention time (min) | UP-SEC Retention time (min) | 0.9652 | <.0001 | 0.9602 | <.0001 |
| Nano-DSF Tm1 (°C) | Nano-DSF Tonset (°C) | 0.9461 | <.0001 | 0.9330 | <.0001 |
| UP-SEC Low pH Retention time (min) | HIC Retention time (min) | 0.8914 | <.0001 | 0.7963 | <.0001 |
| HIC Retention time (min) | UP-SEC Retention time (min) | 0.8307 | <.0001 | 0.6915 | <.0001 |
| Nano-DSF Tagg (°C) | Nano-DSF Tm1 (°C) | 0.7054 | <.0001 | 0.7028 | <.0001 |
| Nano-DSF Tagg (°C) | Nano-DSF Tonset (°C) | 0.6248 | <.0001 | 0.6377 | <.0001 |
| IgG4 | |||||
| AC-SINS PBS pH 7.4 | Nano-DSF Tagg (°C) | 0.7820 | <.0001 | 0.8076 | <.0001 |
| UP-SEC Low pH (% main) | UP-SEC (% main) | 0.7651 | <.0001 | 0.7588 | <.0001 |
| AC-SINS PBS pH 7.4 | Nano-DSF Tonset (°C) | 0.7567 | <.0001 | 0.6716 | <.0001 |
| Nano-DSF Tagg (°C) | Nano-DSF Tm1 (°C) | 0.7221 | <.0001 | 0.8714 | <.0001 |
| Nano-DSF Tagg (°C) | Nano-DSF Tonset (°C) | 0.7130 | <.0001 | 0.7940 | <.0001 |
| cIEF pI | AC-SINS PBS pH 7.4 | 0.7073 | <.0001 | 0.7486 | <.0001 |
| cIEF pI | Nano-DSF Tagg (°C) | 0.6391 | <.0001 | 0.7767 | <.0001 |
| UP-SEC Low pH (% main) | Nano-DSF Tm1 (°C) | 0.6176 | <.0001 | 0.5171 | <.0001 |
| AC-SINS PBS pH 7.4 | AC-SINS NaAc pH 5.5 | 0.6130 | <.0001 | 0.4763 | <.0001 |
| AC-SINS NaAc pH 5.5 | Nano-DSF Tonset (°C) | 0.6093 | <.0001 | 0.5741 | <.0001 |
| cIEF pI | Nano-DSF Tm1 (°C) | 0.5623 | <.0001 | 0.6879 | <.0001 |
| AC-SINS PBS pH 7.4 | Nano-DSF Tm1 (°C) | 0.5553 | <.0001 | 0.5881 | <.0001 |
| Nano-DSF Tm1 (°C) | Nano-DSF Tonset (°C) | 0.5347 | <.0001 | 0.7233 | <.0001 |
| Nano-DSF Tm1 (°C) | UP-SEC (% main) | 0.5303 | <.0001 | 0.3116 | 0.0049 |
| UP-SEC Low pH (% main) | AC-SINS PBS pH 7.4 | 0.5261 | <.0001 | 0.4410 | <.0001 |
| UP-SEC Low pH (% main) | Nano-DSF Tagg (°C) | 0.5160 | <.0001 | 0.4928 | <.0001 |
| HIC Retention time (min) | Nano-DSF Tonset (°C) | 0.5127 | <.0001 | 0.4644 | <.0001 |
| AC-SINS PBS pH 7.4 | HIC Retention time (min) | 0.5071 | <.0001 | 0.6629 | <.0001 |
| UP-SEC Low pH Retention time (min) | HIC Retention time (min) | 0.5066 | <.0001 | 0.5711 | <.0001 |