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. 2020 Apr 13;15(4):e0231131. doi: 10.1371/journal.pone.0231131

Fig 3. Oxidative Stress Index (OSI) in broiler hens fed with different concentrations of GSE dietary supplementation.

Fig 3

Blood samples were collected at 4, 12, 21 and 24 weeks, before diet distribution. The OSI was calculated by the Total Oxidant Status (TOS) divided by the Total Antioxidant Status (TAS) A: animals fed with control diet without GSE supplementation (n = 10), B and C: animals supplemented with GSE at 0.5% and 1% of the total diet composition, respectively, starting at 4 week-old until 40 week-old (n = 10) and D: supplementation at 1% of the total diet composition starting at hatch until 40 week-old (n = 10). Results are presented as lsmeans ± SEM. P values of the effects of the stage when the supplementation is applied (TimeSuppl) and diet were considered as significant if P < 0.05. Different individual letters (a, b and c) indicate a significant effect of the diet. **** P < 0.0001 (diet effect).