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. 2020 Apr 13;14(4):e0008217. doi: 10.1371/journal.pntd.0008217

Fig 4. After intrathoracic injection, ZIKV and USUV can fully disseminate in Ae. japonicus.

Fig 4

Ae. japonicus mosquitoes were intrathoracically injected with ZIKV or USUV. Ae. aegypti mosquitoes, injected with ZIKV, were included as a positive control. After injection, mosquitoes were incubated at 28°C for 14 days. The percentage of virus-positive bodies, legs and wings (L + W) and salivas out of the total number of mosquitoes tested (number positive (+) / total number tested (n)) was determined for (A) Ae. japonicus mosquitoes injected with 4.4 x 103 TCID50 of ZIKV, (B) Ae. japonicus mosquitoes injected with 1.0 x 104 TCID50 of ZIKV, (C) Ae. aegypti mosquitoes injected with ZIKV and (D) Ae. japonicus mosquitoes injected with USUV.