Fig 7. rTsCstN suppressed inflammation mediated by LPS-stimulated mBMDMs.

Transcription levels of IL-1β, IFN-γ, TNF-α, and iNOS were downregulated when LPS-stimulated mBMDMs were treated with rTsCstN after incubation for 12 and 24 h. Irrelevant control (rmDHFR) could not downregulate IL-1β, IFN-γ, TNF-α, and iNOS levels mediated by LPS-stimulated mBMDMs. rTsCstN did not affect the level of anti-inflammatory cytokines and M2 marker (S5 Fig). Negative control (NC) was the medium alone. The results are expressed as mean ± SD. The experiments were performed in triplicate with three independent experiments. One-way ANOVA followed by a Bonferroni multiple comparison test were used for analysis: *p < 0.05, **p < 0.01, and ***p < 0.001, represent differences of cytokine mRNA between LPS-stimulated mBMDM treated with rTsCstN and LPS-stimulated-only mBMDMs.