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. 2020 Apr 1;14(4):e0008192. doi: 10.1371/journal.pntd.0008192

Fig 9. mBMDMs were cultured with or without rTsCstN for 24 h and subsequently stimulated with LPS for 12 h.

Fig 9

Treatment with rTsCstN suppressed mRNA expression of MHC-II, but not its costimulatory molecules (CD80 and CD86) (A). Surface expression of MHC-II, CD80, and CD86 of and costimulatory molecules (CD80 and CD86) on LPS-stimulated mBMDMs was determined by flow cytometry. FACS histograms from one representative experiment suggested that rTsCstN could interfere with surface expression of MHC-II, but not that of CD80 and CD86 (B). The percentage of MHC-II, CD80, or CD86 positive cells on the mBMDMs was calculated, and this emphasized the impact of rTsCstN on suppression of MHC-II expression (C). The data were determined by Flowjo software (TreeStar, Ashland, OR). Two of the three experiments were presented in the form of the percentage of positive cells. Treatment with irrelevant control (rmDHFR) showed a negative result. The results are expressed as mean ± SD. The experiments were performed in triplicate with three independent experiments. One-way ANOVA followed by a Bonferroni multiple comparison test were used for analysis: *p < 0.05, **p < 0.01, and ***p < 0.001, represent differences between LPS-stimulated mBMDM treated with rTsCstN and LPS-stimulated-only mBMDMs.