Table 1. Identification of proteins in ES-L1 fractions 5–8 using Nano-LC/MS/MS.
The experiments were performed in triplicate.
| Fraction no. | Protein name | Accession no. | Theoretical b | MS c score | No. matched peptide d | |
|---|---|---|---|---|---|---|
| Mw | pI | |||||
| 5 | Conserved hypothetical protein a (Tsp_00135) | gi|339239909 | 119.2 | 6.18 | 15 | 1 |
| 6 | Conserved hypothetical protein a (Tsp_04814) | gi|339258426 | 13.2 | 4.54 | 328 | 4 |
| 7 | Conserved hypothetical protein a (Tsp_04814) | gi|339258426 | 13.2 | 4.54 | 158 | 3 |
| ATP-dependent RNA helicase DDX19B | gi|339241083 |
14.9 | 4.6 | 265 | 1 | |
| 8 | ATP-dependent RNA helicase DDX19B | gi|339241083 |
14.8 | 4.6 | 198 | 2 |
| Hypothetical protein a (Tsp_04680) | gi|339258142 | 36.3 | 4.75 | 181 | 2 | |
| Conserved hypothetical protein a (Tsp_16075) | gi|339255022 | 13.9 | 4.75 | 48 | 1 | |
| Antigen targeted by protective antibodies | gi|404638 | 31.2 | 4.76 | 135 | 2 | |
a Locus tag of protein
b Theoretical molecular mass (kDa) and isoelectric point (pI).
c A MS score obtained from MASCOT search results of >70 was used to assign identity to a protein.
d Number of matched peptide was the significant matched protein sequence.