Sperm-defective phenotype of sacy-1(tn1480) at 25°. Dissected gonads of wild-type (A–E) and sacy-1(tn1480) (F–J) stained with anti-MSP antibodies (B and G) and DAPI to detect DNA (C and H). Merged images are also shown (A, D, E, F, I, and J). At 25°, sacy-1(tn1480) hermaphrodites produce swollen and abnormal sperm, which are incapable of fertilization. Note that defective sperm are also found near the bend region in sacy-1(tn1480) adults (F) indicating that there is a defect in the sperm-to-oocyte switch. Bar, 50 μm.