Table 1. RNAi of sacy-1 enhancer loci increases the penetrance of germline or lethal phenotypes in sacy-1(tn1385) reduction-of-function mutants.
| RNAia | Genotype | Sterileb (%) | Gamete degenerationb (%) | Embryonic lethalc (%) |
|---|---|---|---|---|
| L4440 (control) | Wild type | 0 (n = 338) | 0 (n = 338) | 1 (n = 674) |
| sacy-1(tn1385) | 0 (n = 256) | 0 (n = 256) | 1 (n = 502) | |
| mog-2 (II-3D16) | Wild type | 4 (n = 172) | 0 (n = 54) | 7 (n = 574) |
| sacy-1(tn1385) | 43 (n = 254) | 47 (n = 72) | 95 (n = 426) | |
| Y111B2A.25 (III-6G22) | Wild type | 22 (n = 230) | 1 (n = 94) | 84 (n = 463) |
| sacy-1(tn1385) | 95 (n = 272) | 16 (n = 110) | 93 (n = 42) | |
| cacn-1d (II-9E09) | Wild type | 100 (n = 164) | 35 (n = 40) | ND |
| sacy-1(tn1385) | 100 (n = 224) | 81 (n = 52) | ND | |
| emb-4 (V-12E12) | Wild type | 2 (n = 184) | 0 (n = 110) | 12 (n = 337) |
| sacy-1(tn1385) | 89 (n = 176) | 42 (n = 82) | ND | |
| emb-4 (V-12E14) | Wild type | 4 (n = 288) | 0 (n = 96) | 13 (n = 421) |
| sacy-1(tn1385) | 91 (n = 202) | 80 (n = 120) | ND | |
| emb-4 (V-12E16) | Wild type | 4 (n = 210) | 0 (n = 96) | 9 (n = 433) |
| sacy-1(tn1385) | 91 (n = 326) | 87 (n = 140) | ND |
ND, not determined.
RNAi clones showing genetic interactions with sacy-1 are listed with the target gene name in italics and the location of the clone in the RNAi library in parentheses. The identity of clones was verified by DNA sequencing.
Sterility and gamete degeneration were scored by DIC microscopy on the first day of adulthood 24 hr post-L4 at 22°. Gonad arms were scored as sterile if they did not produce embryos and exhibited defects in gametogenesis. Number of gonad arms scored is reported.
Embryonic lethality was measured by conducting daily egg lays over the reproductive lifespan and determining the number of embryos that failed to hatch by 48 hr after egg laying. The number of embryos scored is reported.
cacn-1 was not initially identified as an enhancer of sacy-1 during the genome-wide RNAi screen because cacn-1(RNAi) results in complete sterility in both sacy-1(tn1385) and wild-type animals. However, we determined that RNAi to the Y111B2A.25 pseudogene likely targets cacn-1 (see text for details).