Table 2. Genetic interactions between sacy-1 and emb-4: enhancement of germline and somatic sacy-1 mutant defects.
| Genotype | Vulval rupture (%) | Sterile and Pvla (%) | Sterilea (%) | Fertile (%) |
|---|---|---|---|---|
| sacy-1(tm5503) (n = 284) | 3 | 1 | 96 | 0 |
| sacy-1(tm5503)/+; emb-4(sa44)b (n = 205) | 0 | 0 | 0 | 100 |
| sacy-1(tm5503); emb-4(sa44)b (n = 242) | 83 | 16 | 1 | 0 |
| sacy-1(tn1385) (n = 278) | 0 | 0 | 0 | 100 |
| sacy-1(tn1385)/+; emb-4(sa44)b (n = 201) | 0 | 0 | 0 | 100 |
| sacy-1(tn1385); emb-4(sa44)b,c (n = 144) | 36 | 4 | 3 | 57d |
a
Sterile animals exhibit the sacy-1(lf) gamete degeneration phenotype.
b
The hT2(qIs48) balancer chromosome, which is dominantly marked with GFP, was used to differentiate between sacy-1(tm5503) heterozygotes and homozygotes.
c
The progeny of sacy-1(tn1385)/hT2(qIs48); emb-4(sa44) hermaphrodites; the balancer chromosome provides maternal sacy-1(+) function. The fertile F1 progeny of these animals are maternal-effect lethal, see Table 3.
d
These adult hermaphrodites produce a majority of embryos that fail to hatch, see Table 3.