. 2020 Feb 14;214(4):869–893. doi: 10.1534/genetics.119.302973

Table 5. sacy-1 alleles relevant to this study.

Allele	Alteration	Phenotypes	Inferred activity
Loss-of-function alleles
sacy-1(tm5503)^a	619 bp deletion	Sterile, gamete degeneration	Likely null
sacy-1(tn1615)^b	10 bp deletion	Sterile, gamete degeneration	Likely null
sacy-1(tn1385)^a	G533R	Viable and fertile, suppresses acy-4 sterility, suppresses fog-2 sterility	Reduction of function
sacy-1(tn1391)^a	G473R	Viable and fertile, suppresses acy-4 sterility, suppresses fog-2 sterility	Reduction of function
sacy-1(tn1440)^a	G331R	Viable and fertile, suppresses acy-4 sterility, suppresses fog-2 sterility	Reduction of function
sacy-1(tn1482)^b	D506N	Viable and fertile, suppresses fog-2 sterility^c	Reduction of function
sacy-1(tn1483)^b	G269E	Viable and fertile, suppresses fog-2 sterility^c^,^d	Reduction of function
Alleles with antagonistic activity
sacy-1(tn1479)^b	G504E	Sterile, adult lethal (rupture) or gamete degeneration	Strong loss of function with antimorphic activity
sacy-1(tn1480)^b	H527Y	Viable and fertile at 20° and suppresses fog-2 sterility.^c Embryonic lethal or larval arrest at 15°. Sterile and spermatogenesis-defective at 25°	Loss of function with antimorphic activity, dominant Him
sacy-1(tn1481)^b	P222L	Sterile, masculinization of germline	Recessive gain-of-function

sacy-1(tn1887)^b	R534H	Viable and fertile	Weak antagonistic activity; enhances the dominant Him phenotype of sacy-1(tn1480)

Kim et al. (2012).

This work.

Suppression of acy-4 sterility was not tested.

sacy-1(tn1483) adult hermaphrodites have smaller gonad arms, suggesting effects on germline proliferation (T. Tsukamoto and D. Greenstein, unpublished results).