Table 6. sacy-1(tn1479), sacy-1(tn1480), and sacy-1(tn1481) confer antagonistic activity.
| Allele | Class | Gamete degenerationa | Vulval rupturea | Moga,b | T (°) |
|---|---|---|---|---|---|
| sacy-1(tm5503) (n = 143) | Strong loss-of-function | 5.6 | 94.4 | 0 | 15° |
| sacy-1(tm5503) (n = 96) | Strong loss-of-function | 96.9 | 3.1 | 0 | 20° |
| sacy-1(tm5503) (n = 106) | Strong loss-of-function (from tm5503/+ parents) | 98.0 | 2.0 | 0 | 25° |
| sacy-1(tm5503)c (n = 231) | Strong loss-of-function (from tm5503/tn1480 parents) | 14.7 | 85.3 | 0 | 25° |
| sacy-1(tn1615) (n = 92) | Strong loss-of-function | 97.8 | 2.2 | 0 | 20° |
| sacy-1(tn1479) (n = 170) | Strong loss-of-function with antimorphic activity | 11.2 | 88.8 | 0 | 20° |
| sacy-1(tn1481) (n = 125) | Recessive gain-of-function | 0 | 0 | 100 | 20° |
a
The percentage of adult hermaphrodites exhibiting the reported phenotype is shown. Adults were scored 24 hr post-L4 at 20 or 25° or 48 hr post-L4 at 15°.
b
sacy-1(tn1481) adult hermaphrodites produce large numbers of sperm but no oocytes and are sterile.
c
The sacy-1(tm5503) progeny of sacy-1(tm5503)/unc-13(e1091) sacy-1(tn1480) hermaphrodites grown at 25°.