Table 2. Summary of the PRY and FDY alleles generated by CRISPR.
| Allele | Bases deleted | Bases inserted | Total bases change | Effect | Males that produce progeny at 25° | ||
|---|---|---|---|---|---|---|---|
| # | % | ||||||
| PRY control | — | — | — | — | 20/20 | 100% | Not sterile |
| PRY G5.7 | 1 | 0 | −241 | Out of frame | 10/10 | 100% | Not sterile |
| PRY G54.1 | 244 | 12 | −232 | Out of frame | 9/10 | 90% | Not sterile |
| PRY G54.7 | 259 | 1 | −258 | In frame | 6/10 | 60% | Not sterile |
| PRY G55.8 | 240 | 1 | −239 | Out of frame | 10/10 | 100% | Not sterile |
| PRY G61.2 | 239 | 9 | −230 | Out of frame | 10/10 | 100% | Not sterile |
| FDY control | — | — | — | — | 25/25 | 100% | Not sterile |
| FDY L3.2a | 587 | 0 | −587/−382b | Out of frame | 75/75 | 100% | Not sterile |
| FDY L59.1 | 579 | 0 | −579/−375b | In frame | 10/10 | 100% | Not sterile |
| FDY L64.3 | 581 | 2 | −579/−375b | In frame | 5/5 | 100% | Not sterile |
| FDY L71.4 | 595 | 2 | −593/−383b | Out of frame | 5/5 | 100% | Not sterile |
No alleles of either gene resulted in sterile males.
a
Six independently derived lines had lesions identical to FDY L3.2.
b
Second number represents the total BP change from the start of the coding sequence to the 3′ breakpoint. This number was used for determining if a frameshift occurred.