Table 3. Mutations that disrupt CCY reading frame result in male sterility despite the presence of a wild-type copy of CCY.

	Total flies sequenced	Flies with in-frame indels	Flies with out-of-frame indels	Flies with no double peaksa
Not sterile	14	9	0	5
Sterile	16	0	9	7
Total	30	9	9	12

^aIndels on the free Y in $\widehat{XY}Y$ males resulted in double peaks on sequence traces. A subset of flies, both sterile and nonsterile, had no double peaks and therefore no recognizable lesion.