Table 2. Developmental timing of Spen-KD and CTRL-KD genotypes on diets.
| Diet | CTRL-KD mean (hours AED) | Spen-KD mean (hours AED) | Spen-KD/ CTRL-KD delay (hours) | Two-way ANOVA (P-value) | CTRL-KD (MYD|EXP) chi-square | P-value | Spen-KD (MYD|EXP) chi-square | P-value | Cliff’s delta (d) | |
|---|---|---|---|---|---|---|---|---|---|---|
| Wandering | MYD | 135.5 | 159.5 | 24.0 | <0.0001 | NA | NA | NA | NA | NA |
| HYD | 118.2 | 144.1 | 25.9 | <0.0001 | 208.3 | <0.0001 | 83.47 | <0.0001 | −0.26 | |
| HSD | 139.9 | 162.5 | 22.6 | <0.0001 | 14.95 | 0.0001 | 2.910 | NS | 0.07 | |
| HFD | 136.7 | 164.5 | 27.8 | <0.0001 | 0.7895 | NS | 8.459 | 0.0036 | −0.14 | |
| Pupariation | MYD | 142.6 | 171.5 | 28.9 | <0.0001 | NA | NA | NA | NA | NA |
| HYD | 132.7 | 158.6 | 25.9 | <0.0001 | 77.50 | <0.0001 | 35.55 | <0.0001 | 0.09 | |
| HSD | 142.9 | 168.3 | 25.4 | <0.0001 | 5.153 | 0.0232 | 6.387 | 0.0115 | 0.2 | |
| HFD | 143.6 | 173.2 | 29.6 | <0.0001 | 2.080 | NS | 0.633 | NS | −0.03 | |
| Eclosion | MYD | 239.9 | 272.9 | 33.0 | <0.0001 | NA | NA | NA | NA | NA |
| HYD | 231.1 | 256.2 | 25.1 | <0.0001 | 61.08 | <0.0001 | 52.44 | <0.0001 | 0.2 | |
| HSD | 242.6 | 268.7 | 26.1 | <0.0001 | 10.07 | 0.0015 | 7.247 | 0.0071 | 0.28 | |
| HFD | 243.1 | 280.4 | 37.3 | <0.0001 | 8.648 | 0.0033 | 10.70 | 0.0011 | −0.08 |
Developmental timing is reported in mean hours AED. Cliff’s delta measures the effect size of a diet between two genotypes. Two-way ANOVA compares Spen-KD and CTRL-KD in matched diet conditions. Mean hour after egg deposition (AED). n = 3 replicates, 50 larvae per replicate. Chi-square from log-rank (Mantel–Cox) test.