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. 2020 Jan 27;214(4):927–939. doi: 10.1534/genetics.119.302896

Figure 3.

Figure 3

The β-catenin bar-1 is necessary for anterior V6 lineage seam cell duplications. (A) Anterior V6 lineage seam cell duplications were significantly suppressed in bar-1(ga80) mutants at 25° compared to WT animals grown at 20° (***P < 0.001, binomial test, n ≥ 120). No suppression was seen in egl-20(hu105) and lin-17(n671) mutants, n ≥ 30. (B) The proportion of animals displaying anterior V6 lineage seam cell duplications in WT and mab-5(e1751gf) animals was significantly decreased when grown at 25° on bar-1 RNAi (*P < 0.05 and ***P < 0.001, n ≥ 40). (C) mRNA levels of bar-1, measured by smFISH at the L4 stage. Counts in V6pappa cells are not significantly different between animals grown at 20° and 25° (n ≥ 30). Error bars indicate SE of the proportion (A and B) or mean (C). L4, fourth larval; mRNA, messenger RNA; RNAi, RNA interference; smFISH, single-molecule fluorescence in situ hybridization; WT, wild-type.