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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Fungal Genet Biol. 2020 Mar 3;138:103364. doi: 10.1016/j.fgb.2020.103364

Figure 2. The stability test of transformants.

Figure 2.

A. A picture of an ADE2 gene deletion transformation plate using TRACE. Transformants were grown on YPD plate supplemented with the selection drug. The ade2Δ colonies appear red once the adenine in the media is depleted after several days of growth. B. A diagram shows the replica plating test to screen for stable transformants. Transformants are inoculated onto the master plate and the plate is replicated onto nonselective media for 4 consecutive times. On the fifth passage (P5), cells were replicated onto a selective and a nonselective medium plate. C. The pictures of transformants on a nonselective plate (left) and a selective plate (plate) on passage 5. Transformants that grow robustly on both plates (indicated with black triangles) are considered stable transformants. Those with spotty growth on selective medium are considered non-stable transformants.