. Author manuscript; available in PMC: 2021 May 1.

Published in final edited form as: Fungal Genet Biol. 2020 Mar 3;138:103364. doi: 10.1016/j.fgb.2020.103364

Table 2.

Primer design (deletion of the ADE2 gene in H99)

Gene locus	Primer	Sequence (5’ to 3’)	Purpose
CNAG_02294 ADE2	P1	CTCCGTGTACCACGCTGC	1^st Round of PCR for 5’arm. (pair with P2)
	P2	CTGGCCGTCGTTTTAC-CCACCGCCTGAGGATG	1^st Round of PCR for 5’arm. (pair with PI) Red sequence is overhang complement P7
	P3	GTCATAGCTGTTTCCTG-GTCGACATTCTGGAGGAGG	1^st Round of PCR for 3’arm. (pair with P4) Red sequence is overhang complement P8
	P4	GAAGATGAGGATATTGCCGAAG	1^st Round of PCR for 3’arm. (pair with P3)
	P5	GTGTCGATGGCAGATCTCC	3^rd Round of PCR for full fragment (pair with P6)
	P6	GTCGGTCAAAGGACATCTG	3^rd Round of PCR for full fragment (Pair with P5)
	P7	GTAAAACGACGGCCAG (M13F)	Amplify drug marker, (pair with P8)
	P8	CAGGAAACAGCTATGAC (M13R)	Amplify drug marker, (pair with P7)