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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Hepatology. 2020 Apr 11;72(1):72–87. doi: 10.1002/hep.30990

Figure 1. Increased expression of YAP in Kupffer cells of HFD-fed mice.

Figure 1.

(A) Schematic outline of experimental approaches and analyses. (B) C57BL/6 wild type mice were fed normal chow diet (NCD) or high fat diet (HFD) for 12 weeks (n=6–8 per group). (left panel) Body weight; (mid panel) Liver-to-Body weight ratio; (right panel) H&E staining and YAP Western blotting of liver tissues. (C) Immunohistochemical staining for YAP of liver sections from mice fed NCD or HFD. (D) Immunofluorescence analysis for YAP and F4/80 of liver sections from mice fed NCD or HFD. (E) Expression of YAP in F4/80+ Kupffer cells was measured by ImageStream X in mice fed NCD or HFD (n=4 per group). (F) qRT-PCR analysis for YAP mRNA expression in primary Kupffer cells and hepatocytes obtained from NCD- or HFD-fed mice (n=4 per group). The data are expressed as mean±S.D. ***P<0.001.