Skip to main content
. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Hepatology. 2020 Apr 11;72(1):72–87. doi: 10.1002/hep.30990

Figure 4. LPS/TLR4 signaling induces YAP transcription in Kupffer cells in vitro and in vivo.

Figure 4.

Figure 4.

(A) Primary Kupffer cells isolated from C57/BL6 WT mice were treated with LPS (1 μg/ml) for 24 hrs in 2% FBS containing DMEM medium. The expression of YAP was determined by immunofluorescence and qRT-PCR. (B) WT mice (C57/BL6) were treated with LPS (0.25mg/kg) for 3 weeks (daily i.p. injection) (n=4 per group). The expression of YAP was analyzed by imagestream X (representative images are shown in the left panel). The bar graphs (right panel) represent YAP and TNF-α mRNA levels in primary Kupffer cells isolated from LPS-treated mice. (C) Generation of TLR4ϕKO mice. Deletion of TLR4 was verified by measuring mRNA in Kupffer cells isolated from WT and TLR4ϕKO mice (RT-PCR and qRT-PCR). (D) Effects of LPS on YAP and TNF-α expression in Kupffer cells isolated from WT and TLR4ϕKO mice. The isolated primary Kupffer cells were treated with LPS for 6 hrs. The expression of YAP and TNF-α was determined by qRT-PCR. The data are expressed as mean±S.D. ***P<0.001.