Figure 1. TRPM7 protein is expressed in mouse GV oocytes and MII eggs.
Immunoreactivity of TRPM7 was detected using an anti-TRPM7 + TRPM6 antibody. (A) The immunoreactive band in oocytes and eggs is likely TRPM7 given the ~MW of 210 kDa, and because its functional expression was confirmed by electrophysiology [28]. (B) In all tissues tested here, TRPM6 expression (~MW 240 kDa) appears to be clearly predominant over TRPM7. Red arrows indicate the expected locations of TRPM7 (dark red) and TRPM6 (light red). Representative blots are shown.