Figure 10. TRPM7 channel inhibitors block Ni²⁺ influx.

Inhibition of Ni²⁺ influx (black bars) was examined in the absence/presence of pharmacological inhibitors (dashed bars) and assessed by the rate of fluorescence quenching per minute at F380 relative to baseline fluorescence. Experiments were performed using Mag-Fura-2AM loaded oocytes in divalent free medium. (A) Control, untreated group, (B) Ca_V3.2 inhibitor MBF (1 μM), (C) general inhibitors of TRP channels 2-APB (100 μM) or MgCl₂ (10 mM), (D) TRPM7 inhibitors NS8593 (10 μM) or waixenicin-A (5 μM). (E) Maximal inhibition of fluorescence quenching of Mag-Fura by general TRP inhibitors, and more specifically by the TRPM7 inhibitor NS8593 (P<0.05). (F) Time to inflection measured from the time of addition (5 min) to the time of fluorescence quenching begins, was also maximally inhibited by TRP inhibitors and more specifically by NS8593 (P<0.05). Experiments were replicated 3 times.