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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Cell Calcium. 2020 Feb 22;87:102181. doi: 10.1016/j.ceca.2020.102181

Figure 11. The absence of TRPV3- and V3-CaV3.2 channels reduces internal Ca2+ stores.

Figure 11.

Ca2+ release from ER and total stores was measured using TG (10 μM) and IO (2.5 μM) in WT and in oocytes lacking TRPV3, CaV3.2, or V3-CaV3.2 channels. Experiments were performed in nominal Ca2+-free medium. (A) TG responses in WT, TRPV3-, CaV3.2- and V3-CaV3.2-KO oocytes. (B) Ionomycin responses in WT, TRPV3-, CaV3.2- and V3-CaV3.2-KO oocytes. (C) Peak amplitude of TG-induced Ca2+ release was reduced in CaV3.2 (P<0.05). (D) Peak amplitude of ionomycin-induced Ca2+ release was reduced V3-CaV3.2 (P<0.05), whereas (E) AUC response induced by IO was reduced in all KO lines (P<0.05). Open bars indicate nominal Ca2+ free media; filled gray bars show the time at which TG/IO was added. Experiments were replicated 3 times.