Figure 11. The absence of TRPV3- and V3-Ca_V3.2 channels reduces internal Ca²⁺ stores.

Ca²⁺ release from ER and total stores was measured using TG (10 μM) and IO (2.5 μM) in WT and in oocytes lacking TRPV3, Ca_V3.2, or V3-Ca_V3.2 channels. Experiments were performed in nominal Ca²⁺-free medium. (A) TG responses in WT, TRPV3-, Ca_V3.2- and V3-Ca_V3.2-KO oocytes. (B) Ionomycin responses in WT, TRPV3-, Ca_V3.2- and V3-Ca_V3.2-KO oocytes. (C) Peak amplitude of TG-induced Ca²⁺ release was reduced in Ca_V3.2 (P<0.05). (D) Peak amplitude of ionomycin-induced Ca²⁺ release was reduced V3-Ca_V3.2 (P<0.05), whereas (E) AUC response induced by IO was reduced in all KO lines (P<0.05). Open bars indicate nominal Ca²⁺ free media; filled gray bars show the time at which TG/IO was added. Experiments were replicated 3 times.