Figure 3. Ca²⁺ influx in GV oocytes is mediated by multiple channels.

Presence of spontaneous oscillations and Ca²⁺ influx examined in WT oocytes and oocytes lacking TRPV3, Ca_V3.2 & V3-Ca_V3.2 channels. (A-four upper panels) Spontaneous Ca²⁺ oscillations in WT and KO oocytes in media containing basal Ca²⁺ levels (2 mM). Fewer oocytes of the Ca_V3.2 and V3-Ca_V3.2 KO lines display oscillations, which are also of decreased frequency (D) (P< 0.05). (B-four medium panels) Ca²⁺ influx triggered by increasing [Ca²⁺]_o from 2 mM to 5 mM induces oscillations in all WT and KO lines, which appear of equal initial frequency (E). (C-four lower panels) After addition of TG (10 μM), Ca²⁺ influx resulted in reduced responses in Ca_V3.2 and V3-Ca_V3.2 KO oocytes, especially in the interval from Ca²⁺addition to the 1^st Ca²⁺ peak (P< 0.05) (F). Filled horizontal bars above each panel show the time of addition of Ca²⁺ (black) or TG (grey) to the media. All experiments were 4 replicated for times. Asterisk(s) above columns in bar graphs denote significant differences from other conditions here and throughout the manuscript.