Figure 4. Pharmacological inhibitors disrupt Ca²⁺ influx/oscillations in GV oocytes.

Ca²⁺ influx in GV oocytes was tested by increasing [Ca²⁺]_o from 2- to 5 mM (white/black horizontal bars above each panel) in the presence of pharmacological inhibitors (dashed bars). Increasing [Ca²⁺]_o in (A) control, untreated oocytes, or oocytes treated with (B) Ca_V3.2 inhibitors: MBF (1 μM) or Ni²⁺ (100 μM) induced oscillations in the majority of oocytes. Conversely, Increasing [Ca²⁺]_o in the presence of the (C) general TRP channel inhibitors 2-APB (50 μM) or MgCl₂ (10 mM), or (D) in the presence of the TRPM7 inhibitors NS8593 (10 μM) or waixenicin-A (5 μM) failed to induce oscillations (P <0.05). All experiments were performed in 4 replicates.