Figure 5. Sr²⁺-induced oscillations are diminished in Ca_V3.2- and V3-Ca_V3.2-KO oocytes.

Sr²⁺ induced oscillations were tested in WT and in oocytes lacking TRPV3, or Ca_V3.2 or V3-Ca_V3.2 channels. Experiments were performed in nominal Ca²⁺-free media containing 10 mM Sr²⁺. (A) Sr²⁺ induced the expected oscillations in WT oocytes, and largely similar responses in (B) TRPV3-KO oocytes. Sr²⁺ induced oscillations were reduced in (C) Ca_V3.2-KO and in (D) V3-Ca_V3.2-KO oocytes both in the (E) number of cells showing oscillations, and in the (F) number of rises during the first 1h of imaging (P<0.05). Horizontal bars above each panel denote the time during which Sr²⁺ was present in the media. All experiments were performed in 3–4 replicates.