Figure 7. Multiple channels enable Mn²⁺ entry.

Mn²⁺ influx was tested in WT and in oocytes lacking TRPV3, or Ca_V3.2 or V3-Ca_V3.2 channels. Experiments were performed in media containing 2 mM Ca²⁺. (A) Mn²⁺ influx in WT oocytes, (B) TRPV3-KO oocytes, (C) Ca_V3.2-KO oocytes, (D) V3-Ca_V3.2-KO oocytes. (E) Fluorescence quenching assessed by the rate of F360 nm fluorescence decrease per minute relative to baseline fluorescence (P<0.05). (F) Time to inflection measured from the time of Mn²⁺ addition (5 min) to the time of first significant change in fluorescence values, which shortened in V3-Ca_V3.2-KO oocytes (P<0.05). Black horizontal bars denote addition of 100 μM Mn²⁺. Experiments were replicated 4 times.