Figure 9. Enhanced Ni²⁺ influx in V3-Ca_V3.2-KO oocytes.

Ni²⁺ influx was tested in WT and oocytes lacking TRPV3, Ca_V3.2 or V3-Ca_V3.2 channels. Experiments were performed using Mag-Fura-2AM loaded oocytes in divalent free medium (DFM). Ni²⁺ influx in (A) WT oocytes, (B) TRPV3-KO oocytes, (C) Ca_V3.2-KO oocytes, (D) V3-Ca_V3.2-KO oocytes. (E) Fluorescence quenching at F380 assessed by the rate of fluorescence decrease per minute relative to baseline fluorescence (*P<0.05). (F) Time to inflection measured from the time of addition (5 min) to the time fluorescence quenching begins (*P<0.05). Black bars show the time at which Ni²⁺ was added. Experiments were replicated three times.