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. 2020 Apr 9;13:3061–3071. doi: 10.2147/OTT.S246632

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© 2020 Tian et al.

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RSF-1 siRNA promotes radiation-induced DNA damage. (A) The extent of DNA damage was assessed by Comet assay. The percentage of DNA in comet tail (Tail DNA %) was quantified and graphed for each group. (B) The expression of H2AX and γH2AX proteins were examined by Western blot. The ratio of γH2AX level relative to H2AX after normalization to GAPDH was quantified and graphed for each group. (C) HeLa cells transfected with siRNA-NC or siRNA-RSF-1 were irradiated with 4Gy and then stained with an anti-RSF-1 (green) or an anti-γH2AX (red) antibody. RSF-1- and γH2AX-positively stained cells were scored and showed in right panel. The experiments were repeated three times independently. ***P<0.001, **P<0.01, *P <0.05 as compared with the siRNA-NC group.