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. 2020 Feb 5;48(5):1451–1462. doi: 10.1007/s10439-020-02462-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Time evolution of membrane permeability. Evolution of membrane permeability to PI and Yo-Pro-1 after treatment of cells in suspension with conventional IRE (100 μs monopolar pulses) and 2-5-2 H-FIRE bursts with amplitudes of 1100 and 3800 V/cm, respectively. Squares show the mean percentage of cells permeable to PI and Yo-Pro-1 at different times after treatment (n ≥ 4 for each time point). Error bars show the standard deviation.