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. 2020 Apr 9;13:1033–1044. doi: 10.2147/IDR.S244896

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© 2020 Moosavian et al.

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Comparison of different spa polymorphic region X HRM curves obtained from MRSA isolates. (A) Negative derivative of fluorescence over temperature (df/dt) plots displaying 10 HRM profiles. (B) Normalization data curve shows the decreasing fluorescence vs increasing temperature. (C) Difference graph demonstrating the accurate reproduction of eight spa HRM profiles in a run experiment. Isolates with difference plots that fall within the ±0.2 relative ﬂuorescence unit (RFU) cutoffs were considered as the “same” type, while the isolates that lie outside of the ±0.2 RFU cutoffs were denoted as “different”.