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Identification of BcNR C109 metabolites. Unstained (A) and UV light visualized (B) TLC analyses of the BcNR reaction using C109 as substrate (Rz), compared with the control performed with the same reaction mixture but without NADPH, demonstrated that the enzyme is able to transform C109 compound. The MS analysis [(C) full mass, and (D) fragmentation pattern] of the purified reaction products demonstrated that BcNR catalyzes the reduction of the nitro group of C109.
