FIGURE 2.

(A) Schematic drawing of the embedding protocol using low molecular weight PEGDA (P575) for soft hydrogel scaffolds with high aspect-ratio features. The scaffolds can be re-embedded in routine embedding media and sectioned using either cryosectioning or vibrating microtomy. (B) Image sequence of the different stages of the process. From left to right: a microstructured scaffold fabricated on PET membrane, the P575 block embedding the microstructured scaffold, the P575 block re-embedded in an agarose medium, and a free-standing, robust single section after vibrating microtomy sectioning. Scale bar: 5 mm.