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. 2020 Apr 7;8:294. doi: 10.3389/fbioe.2020.00294

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Copyright © 2020 Altay, Tosi, García-Díaz and Martínez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Bright field microscopy image of villus-like microstructured PEGDA-AA hydrogels stained with Alcian Blue (left panel) and confocal maximum intensity projection image of the PEGDA-AA microstructures functionalized with BSA-Texas Red (right panel) after P575 embedding and sectioning. White dashed lines mark the border of the microstructures. Scale bars: 200 μm. (B) Bright field microscopy image of a vibratome section of the P575 embedded villus-like hydrogel scaffold with Caco-2 cells grown on top. Scale bar: 200 μm. (C) Bright field microscopy images of cryosectioned villus-like hydrogel scaffolds with Caco-2 cells grown on top. The upper panel shows the hydrogel embedded in P575 and then in OCT; the lower panel shows the control only with the OCT embedding. Scale bars: 100 μm.