FIGURE 5.

Fast Airyscan confocal images showing F-actin and nuclei staining of the Caco-2 monolayer formed on top of microstructured hydrogels after 21 days in culture (upper row). Yellow boxed regions are shown in high magnification (middle and lower rows). Different cell shapes are observed: basally constricted wedge-shaped at the villi tips, cuboidal along the walls and apically constricted at the base, as represented by the white drawings. Scale bars (upper and middle rows): 50 μm, (lower row): 30 μm.