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Fast Airyscan confocal images of the Caco-2 monolayer formed on top of the microstructured hydrogels after 21 days in culture showing the expression of (A) villin in green or (B) ZO-1 in yellow, β-catenin in magenta, and nuclei in blue. Yellow boxed regions are shown in high magnification. Scale bars (upper and middle rows): 50 μm, (lower row): 20 μm.
